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antibodies for activation and cytokine neutralization  (Bio X Cell)


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    Bio X Cell antibodies for activation and cytokine neutralization
    ( A ) Naïve CD4 + T cells from WT or KO mice were differentiated into T H 17 cells (as described in Materials and Methods) for 4 days, and intracellular cytokines were scored by flow cytometry. The left panel is a representative flow figure showing frequency of IL-17A + and IFN-γ + cells, and the right panel shows the cumulative data of the same. n = 5 biologically independent samples, representative of five experiments. ( B ) iMFI of IL-17A. iMFI = (MFI)( P ) where, MFI is the median fluorescence intensity of <t>cytokine-positive</t> cells, and P is the percentage of cytokine-positive cells. ( C ) WT and KO naïve T cells were differentiated under T H 17 conditions for 4 days and reactivated with anti-CD3 for 24 hours, and the culture supernatant was analyzed for IL-17A by bioplex assay. n = 3 biologically independent samples. ( D ) WT and KO naïve T cells were differentiated into T H 17 cells for 3 days and RNA quantified by reverse transcription qPCR. n = 3 to 5 biologically independent samples. ( E and F ) WT and KO naïve CD4 + T cells were differentiated into T H 17 cells with IL-1β + IL-6 + IL-23 (E) or TGF-β1 + IL1-β + IL-6 + IL-23 (F) for 4 days and analyzed for frequency of IL-17A + and IFN-γ + cells by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data for the same. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.
    Antibodies For Activation And Cytokine Neutralization, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+activation+cytokine+neutralization/pmc11042737-289-4-9?v=Bio+X+Cell
    Average 90 stars, based on 1 article reviews
    antibodies for activation and cytokine neutralization - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Sphingolipid biosynthesis is essential for metabolic rewiring during T H 17 cell differentiation"

    Article Title: Sphingolipid biosynthesis is essential for metabolic rewiring during T H 17 cell differentiation

    Journal: Science Advances

    doi: 10.1126/sciadv.adk1045

    ( A ) Naïve CD4 + T cells from WT or KO mice were differentiated into T H 17 cells (as described in Materials and Methods) for 4 days, and intracellular cytokines were scored by flow cytometry. The left panel is a representative flow figure showing frequency of IL-17A + and IFN-γ + cells, and the right panel shows the cumulative data of the same. n = 5 biologically independent samples, representative of five experiments. ( B ) iMFI of IL-17A. iMFI = (MFI)( P ) where, MFI is the median fluorescence intensity of cytokine-positive cells, and P is the percentage of cytokine-positive cells. ( C ) WT and KO naïve T cells were differentiated under T H 17 conditions for 4 days and reactivated with anti-CD3 for 24 hours, and the culture supernatant was analyzed for IL-17A by bioplex assay. n = 3 biologically independent samples. ( D ) WT and KO naïve T cells were differentiated into T H 17 cells for 3 days and RNA quantified by reverse transcription qPCR. n = 3 to 5 biologically independent samples. ( E and F ) WT and KO naïve CD4 + T cells were differentiated into T H 17 cells with IL-1β + IL-6 + IL-23 (E) or TGF-β1 + IL1-β + IL-6 + IL-23 (F) for 4 days and analyzed for frequency of IL-17A + and IFN-γ + cells by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data for the same. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.
    Figure Legend Snippet: ( A ) Naïve CD4 + T cells from WT or KO mice were differentiated into T H 17 cells (as described in Materials and Methods) for 4 days, and intracellular cytokines were scored by flow cytometry. The left panel is a representative flow figure showing frequency of IL-17A + and IFN-γ + cells, and the right panel shows the cumulative data of the same. n = 5 biologically independent samples, representative of five experiments. ( B ) iMFI of IL-17A. iMFI = (MFI)( P ) where, MFI is the median fluorescence intensity of cytokine-positive cells, and P is the percentage of cytokine-positive cells. ( C ) WT and KO naïve T cells were differentiated under T H 17 conditions for 4 days and reactivated with anti-CD3 for 24 hours, and the culture supernatant was analyzed for IL-17A by bioplex assay. n = 3 biologically independent samples. ( D ) WT and KO naïve T cells were differentiated into T H 17 cells for 3 days and RNA quantified by reverse transcription qPCR. n = 3 to 5 biologically independent samples. ( E and F ) WT and KO naïve CD4 + T cells were differentiated into T H 17 cells with IL-1β + IL-6 + IL-23 (E) or TGF-β1 + IL1-β + IL-6 + IL-23 (F) for 4 days and analyzed for frequency of IL-17A + and IFN-γ + cells by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data for the same. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Techniques Used: Flow Cytometry, Fluorescence, BioPlex Assay, Reverse Transcription

    ( A ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and scored for phospho-Akt(S473) by flow cytometry. The left panel is a representative flow figure, and the right panel shows cumulative p-Akt MFI. n = 3 biologically independent samples. ( B ) GSEA for positive regulation of ROS genes between WT and KO T H 17 cells. ( C ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and intracellular ROS measured using DCFDA by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data of DCFDA MFI. n = 3 biologically independent samples. ( D ) WT and KO naïve cells were differentiated under T H 17 conditions for 4 days with or without NAC and scored for intracellular cytokine. The left panel is a representative flow figure, and the right panel shows the cumulative data of the same. n = 4 to 6 biologically independent samples. ( E ) WT and KO naïve cells were differentiated under T H 17 conditions with or without NAC for 12 hours and scored for frequency of phospho-S6 + cells by flow cytometry. n = 5 biologically independent samples. ( F to I ) GSEA for (F) mTOR signaling genes, (G) c-Myc target genes, (H) HIF-1α target genes, and (I) glycolytic genes KO and (WT and KO + NAC) T H 17 cells. n = 3 biologically independent samples. ( J ) Hk2 mRNA quantification by qPCR of T H 17 under the indicated differentiation conditions. n = 3 biologically independent samples. ( K ) WT and KO naïve T cells were differentiated under T H 17 conditions with or without NAC for 3 days and ECAR was measured in equal number of viable cells by Seahorse analyzer. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.
    Figure Legend Snippet: ( A ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and scored for phospho-Akt(S473) by flow cytometry. The left panel is a representative flow figure, and the right panel shows cumulative p-Akt MFI. n = 3 biologically independent samples. ( B ) GSEA for positive regulation of ROS genes between WT and KO T H 17 cells. ( C ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and intracellular ROS measured using DCFDA by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data of DCFDA MFI. n = 3 biologically independent samples. ( D ) WT and KO naïve cells were differentiated under T H 17 conditions for 4 days with or without NAC and scored for intracellular cytokine. The left panel is a representative flow figure, and the right panel shows the cumulative data of the same. n = 4 to 6 biologically independent samples. ( E ) WT and KO naïve cells were differentiated under T H 17 conditions with or without NAC for 12 hours and scored for frequency of phospho-S6 + cells by flow cytometry. n = 5 biologically independent samples. ( F to I ) GSEA for (F) mTOR signaling genes, (G) c-Myc target genes, (H) HIF-1α target genes, and (I) glycolytic genes KO and (WT and KO + NAC) T H 17 cells. n = 3 biologically independent samples. ( J ) Hk2 mRNA quantification by qPCR of T H 17 under the indicated differentiation conditions. n = 3 biologically independent samples. ( K ) WT and KO naïve T cells were differentiated under T H 17 conditions with or without NAC for 3 days and ECAR was measured in equal number of viable cells by Seahorse analyzer. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Techniques Used: Flow Cytometry



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    ( A ) Naïve CD4 + T cells from WT or KO mice were differentiated into T H 17 cells (as described in Materials and Methods) for 4 days, and intracellular cytokines were scored by flow cytometry. The left panel is a representative flow figure showing frequency of IL-17A + and IFN-γ + cells, and the right panel shows the cumulative data of the same. n = 5 biologically independent samples, representative of five experiments. ( B ) iMFI of IL-17A. iMFI = (MFI)( P ) where, MFI is the median fluorescence intensity of <t>cytokine-positive</t> cells, and P is the percentage of cytokine-positive cells. ( C ) WT and KO naïve T cells were differentiated under T H 17 conditions for 4 days and reactivated with anti-CD3 for 24 hours, and the culture supernatant was analyzed for IL-17A by bioplex assay. n = 3 biologically independent samples. ( D ) WT and KO naïve T cells were differentiated into T H 17 cells for 3 days and RNA quantified by reverse transcription qPCR. n = 3 to 5 biologically independent samples. ( E and F ) WT and KO naïve CD4 + T cells were differentiated into T H 17 cells with IL-1β + IL-6 + IL-23 (E) or TGF-β1 + IL1-β + IL-6 + IL-23 (F) for 4 days and analyzed for frequency of IL-17A + and IFN-γ + cells by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data for the same. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.
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    Image Search Results


    ( A ) Naïve CD4 + T cells from WT or KO mice were differentiated into T H 17 cells (as described in Materials and Methods) for 4 days, and intracellular cytokines were scored by flow cytometry. The left panel is a representative flow figure showing frequency of IL-17A + and IFN-γ + cells, and the right panel shows the cumulative data of the same. n = 5 biologically independent samples, representative of five experiments. ( B ) iMFI of IL-17A. iMFI = (MFI)( P ) where, MFI is the median fluorescence intensity of cytokine-positive cells, and P is the percentage of cytokine-positive cells. ( C ) WT and KO naïve T cells were differentiated under T H 17 conditions for 4 days and reactivated with anti-CD3 for 24 hours, and the culture supernatant was analyzed for IL-17A by bioplex assay. n = 3 biologically independent samples. ( D ) WT and KO naïve T cells were differentiated into T H 17 cells for 3 days and RNA quantified by reverse transcription qPCR. n = 3 to 5 biologically independent samples. ( E and F ) WT and KO naïve CD4 + T cells were differentiated into T H 17 cells with IL-1β + IL-6 + IL-23 (E) or TGF-β1 + IL1-β + IL-6 + IL-23 (F) for 4 days and analyzed for frequency of IL-17A + and IFN-γ + cells by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data for the same. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Journal: Science Advances

    Article Title: Sphingolipid biosynthesis is essential for metabolic rewiring during T H 17 cell differentiation

    doi: 10.1126/sciadv.adk1045

    Figure Lengend Snippet: ( A ) Naïve CD4 + T cells from WT or KO mice were differentiated into T H 17 cells (as described in Materials and Methods) for 4 days, and intracellular cytokines were scored by flow cytometry. The left panel is a representative flow figure showing frequency of IL-17A + and IFN-γ + cells, and the right panel shows the cumulative data of the same. n = 5 biologically independent samples, representative of five experiments. ( B ) iMFI of IL-17A. iMFI = (MFI)( P ) where, MFI is the median fluorescence intensity of cytokine-positive cells, and P is the percentage of cytokine-positive cells. ( C ) WT and KO naïve T cells were differentiated under T H 17 conditions for 4 days and reactivated with anti-CD3 for 24 hours, and the culture supernatant was analyzed for IL-17A by bioplex assay. n = 3 biologically independent samples. ( D ) WT and KO naïve T cells were differentiated into T H 17 cells for 3 days and RNA quantified by reverse transcription qPCR. n = 3 to 5 biologically independent samples. ( E and F ) WT and KO naïve CD4 + T cells were differentiated into T H 17 cells with IL-1β + IL-6 + IL-23 (E) or TGF-β1 + IL1-β + IL-6 + IL-23 (F) for 4 days and analyzed for frequency of IL-17A + and IFN-γ + cells by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data for the same. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Article Snippet: Antibodies for activation and cytokine neutralization were procured from Bio X Cell, USA.

    Techniques: Flow Cytometry, Fluorescence, BioPlex Assay, Reverse Transcription

    ( A ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and scored for phospho-Akt(S473) by flow cytometry. The left panel is a representative flow figure, and the right panel shows cumulative p-Akt MFI. n = 3 biologically independent samples. ( B ) GSEA for positive regulation of ROS genes between WT and KO T H 17 cells. ( C ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and intracellular ROS measured using DCFDA by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data of DCFDA MFI. n = 3 biologically independent samples. ( D ) WT and KO naïve cells were differentiated under T H 17 conditions for 4 days with or without NAC and scored for intracellular cytokine. The left panel is a representative flow figure, and the right panel shows the cumulative data of the same. n = 4 to 6 biologically independent samples. ( E ) WT and KO naïve cells were differentiated under T H 17 conditions with or without NAC for 12 hours and scored for frequency of phospho-S6 + cells by flow cytometry. n = 5 biologically independent samples. ( F to I ) GSEA for (F) mTOR signaling genes, (G) c-Myc target genes, (H) HIF-1α target genes, and (I) glycolytic genes KO and (WT and KO + NAC) T H 17 cells. n = 3 biologically independent samples. ( J ) Hk2 mRNA quantification by qPCR of T H 17 under the indicated differentiation conditions. n = 3 biologically independent samples. ( K ) WT and KO naïve T cells were differentiated under T H 17 conditions with or without NAC for 3 days and ECAR was measured in equal number of viable cells by Seahorse analyzer. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Journal: Science Advances

    Article Title: Sphingolipid biosynthesis is essential for metabolic rewiring during T H 17 cell differentiation

    doi: 10.1126/sciadv.adk1045

    Figure Lengend Snippet: ( A ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and scored for phospho-Akt(S473) by flow cytometry. The left panel is a representative flow figure, and the right panel shows cumulative p-Akt MFI. n = 3 biologically independent samples. ( B ) GSEA for positive regulation of ROS genes between WT and KO T H 17 cells. ( C ) WT and KO naïve cells were differentiated under T H 17 conditions for 12 hours and intracellular ROS measured using DCFDA by flow cytometry. The left panel is a representative flow figure, and the right panel shows the cumulative data of DCFDA MFI. n = 3 biologically independent samples. ( D ) WT and KO naïve cells were differentiated under T H 17 conditions for 4 days with or without NAC and scored for intracellular cytokine. The left panel is a representative flow figure, and the right panel shows the cumulative data of the same. n = 4 to 6 biologically independent samples. ( E ) WT and KO naïve cells were differentiated under T H 17 conditions with or without NAC for 12 hours and scored for frequency of phospho-S6 + cells by flow cytometry. n = 5 biologically independent samples. ( F to I ) GSEA for (F) mTOR signaling genes, (G) c-Myc target genes, (H) HIF-1α target genes, and (I) glycolytic genes KO and (WT and KO + NAC) T H 17 cells. n = 3 biologically independent samples. ( J ) Hk2 mRNA quantification by qPCR of T H 17 under the indicated differentiation conditions. n = 3 biologically independent samples. ( K ) WT and KO naïve T cells were differentiated under T H 17 conditions with or without NAC for 3 days and ECAR was measured in equal number of viable cells by Seahorse analyzer. n = 3 biologically independent samples. Each dot represents an individual mouse. All data presented as means ± SEM: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant.

    Article Snippet: Antibodies for activation and cytokine neutralization were procured from Bio X Cell, USA.

    Techniques: Flow Cytometry